1. To develop new methods of fluorescent benzanthrone derivative synthesis and to characterize the obtained compounds

Tasks of the research activity:

1. To perform analysis of academic literature and patents.

2.     To synthesize benzanthrone derivatives with hydrophobic and hydrophilic substitutes 

3.     To characterize physical and chemical properties of the obtained substances. 

In the beginning of commencement of the activity and also in later stages, the latest academic works and patents will be analyzed.

Under this activity it is planned to synthesize new compounds of benzanthrone that are not described in literature with explicitly luminescent properties expected. In previous tasks so far we have synthesized strongly hydrophobic derivatives; such compounds are widely used in research of lipophyle environment. It is also important to develop fluorescent probes that dissolve in hydrophilic environment and, due to this, become usable in research of proteins and nucleic acids. We plan to obtain such substances by developing methods of synthesis with quaternary ammonium substitute – introduction of pyridinium, quinolinium chloride, and other residues into benzanthrone system. The obtained quaternary ammonium ions are able to develop salts with nucleic acids and in compounded means properties of fluorescence will certainly depend on the surrounding environment, namely, on conformations of nucleic acids and other changes in the structure thereof. Therefore the newly synthesized hydrophilic fluorophores will be used in activities 5 – 7 for development of fluorescent methods.

Another planned direction is synthesis of compounds luminescent in infrared range. Such substances are perspective both in research of biologic objects and in design of new, optical materials for activity No. 4. It is planned to be achieved by introduction of various substituted phenoxazine and porphyrin residues into molecule of benzanthrone.

After procedures of new substance synthesis and separation, structure demonstration will be performed with accepted standard methods (NMR, IR, mass-spectra), as well as determination of physical and chemical properties. 

Laboratory premises of Department of Chemistry, G. Liberts Innovative Microscopy Centre of Daugavpils University and the following academic equipment will be used in work (Gas Chromatograph Quadrupole Mass Spectrometer Shimadzu QP2010, spectrophotometer “CECIL 1021”, x-ray difractometer RIGAKU).